Part:BBa_K5490002

Inducible endonuclease targets RNA via gRNA, contains an HA tag

CasRx is a highly effective RNA-targeting molecule with nuclease activity specifically against single-stranded RNA, making it a versatile tool for gene silencing and antiviral therapies. One of its major advantages over RNA interference (RNAi) is its lower off-target activity, providing more precision in targeting specific RNA sequences. As a viral protein, CasRx operates independently of the host's cellular machinery, which reduces variability and enhances efficiency.

CasRx is the smallest member of the RNA-targeting CRISPR family, allowing for easier delivery into cells while maintaining high specificity and efficiency in cleaving target RNA. Its minimal off-target effects and high efficiency make it ideal for large-scale transcriptome screening, precise gene silencing, and targeting viral RNA in therapeutic applications.

A key advantage of CasRx over other CRISPR systems is its simplicity--it does not require a PAM sequence or a tracrRNA, making it one of the most straightforward CRISPR systems to implement. CasRx can target virtually any RNA sequence when paired with a guide RNA (gRNA) that is complementary to the target.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 499
  Illegal EcoRI site found at 919
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 499
  Illegal EcoRI site found at 919
  Illegal NotI site found at 2908
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 499
  Illegal EcoRI site found at 919
  Illegal BamHI site found at 2899
  Illegal XhoI site found at 2374
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 499
  Illegal EcoRI site found at 919
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 499
  Illegal EcoRI site found at 919
  Illegal NgoMIV site found at 187
  
• 1000
  COMPATIBLE WITH RFC[1000]